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BALB/3T3 clone A31
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S Aaronson
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Mus musculus (mouse)
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fibroblast
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Organ: embryo
Strain: BALB/c
Cell Type: fibroblast
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation date: 1968
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testing [92346] [92389]
transfection host (Roche FuGENE® Transfection Reagents)
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Herpes simplex virus
Vesicular stomatitis virus
Human poliovirus 1
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No
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negative
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modal number = 78; range = 62 to 109.
The stemline number is hypotetraploid. Most of the cells contained only telocentric or acrocentric chromosomes.
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embryo; 14 to 17 day gestation
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: The serum used is calf serum, NOT fetal calf serum. The depositor recommended calf serum because fetal calf serum causes transformation and loss of contact inhibition
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