CCL-97 R2C 大鼠睪丸間質細胞瘤

ATCC® Number:	CCL-97?	Price:	$438.00
Designations: R2C Depositors:  G Sato Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Rattus norvegicus (rat) Morphology: epithelial Source: Strain: WFu Organ: testis Disease: Leydig cell tumor Cellular Products: steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.   Applications: transfection host (Roche FuGENE® Transfection Reagents) Virus Resistance: poliovirus 2 Reverse Transcript: positive Age: 2 months Gender: male Comments: Stimulation by other hormones or cAMP is not required for hormone secretion. Propagation: ATCC complete growth medium: The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5% horse serum to a final concentration of 15% Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Add fresh medium at least twice per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Preservation: culture medium 95%; DMSO, 5% Related Products: recommended serum:ATCC 30-2020 recommended serum:ATCC 30-2040 References: 1100: Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022

ATCC® Number: CCL-97? Price: $438.00
Designations: R2C
Depositors: G Sato
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus (rat)
Morphology: epithelial


Source: Strain: WFu
Organ: testis
Disease: Leydig cell tumor
Cellular Products: steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Roche FuGENE® Transfection Reagents)
Virus Resistance: poliovirus 2
Reverse Transcript: positive
Age: 2 months
Gender: male
Comments: Stimulation by other hormones or cAMP is not required for hormone secretion.
Propagation: ATCC complete growth medium: The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium:
?fetal bovine serum to a final concentration of 2.5%
?horse serum to a final concentration of 15%

Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Add fresh medium at least twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 1100: Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022