EBTr (NBL-4) 牛胚氣管細胞
ATCC® Number:  CCL-44?      
Designations:  EBTr (NBL-4) 
Depositors:   AJ Kniazeff, WA Nelson-Rees, NB Darby 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Bos taurus (cow) 
Morphology: fibroblast

 
Source: Organ: trachea
Disease: normal
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Virus Susceptibility: bovine viral diarrhea; infectious bovine rhinotracheitis; parainfluenzavirus 3; herpes simplex; reovirus 3; vesicular stomatitis (Ogden); vaccinia
Virus Resistance: human adenovirus 5; poliovirus 2; coxsackievirus A9, B5 
Reverse Transcript: negative 
Age:  embryo 
Gender:  male 
Comments: The cells are negative for bovine viral diarrhea virus (BVDV). [22883]
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:  Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
27802: Vassilev VB, et al. Authentic and chimeric full-length genomic cDNA clones of bovine viral diarrhea virus that yield infectious transcripts. J. Virol. 71: 471-478, 1997. PubMed: 8985373