CCL-119 CCRF-CEM 人急性細(xì)胞白血病T細(xì)胞

ATCC® Number:  CCL-119?     
Designations:  CCRF-CEM [CCRF CEM] 
Depositors:   GE Foley 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: suspension
Organism: Homo sapiens (human) 
Morphology: lymphoblast

 
Source: Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: T lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Isolation:  Isolation date: November, 1964
Applications: transfection host (Nucleofection technology from Lonza)
Tumorigenic: Yes 
Reverse Transcript: negative 
Antigen Expression: CD3; Homo sapiens, expressed
CD4; Homo sapiens, expressed
CD5; Homo sapiens, expressed
CD7; Homo sapiens, expressed
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 10,13
D5S818: 12,13
D7S820: 9,13
THO1: 6,7
TPOX: 8
vWA: 17,19
Cytogenetic Analysis: modal number = 47; range = 41 to 95.
Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twenty-eight percent of the cells with 45 chromosomes were C-; 53% of all cells had an extra D and 35% had an extra F. Only N1 and N18 were not affected by gain or loss. No marker chromosomes noted.
Isoenzymes:  ADA, 1
ES-D, 1
G6PD, B
GLO-I, 1
PEP-D, 1
PGD, C
PGM1, 1
PGM3, 0
Age:  4 years juvenile 
Gender:  female 
Ethnicity:  Caucasian 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:  Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml. Maintain cell density between 2 to 3 X 10 exp5 and 1 to 2 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CRL-2264
derivative:ATCC CRL-2265
derivative:ATCC CRL-8993
derivative:ATCC TIB-195
References: 22788: Foley GE, et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965. PubMed: 14278051
23337: Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323
25967: Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381
26284: Uzman BG, et al. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725-1742, 1966. PubMed: 5224274
26293: Adams RA, et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. PubMed: 4295047
32913: Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538