A9 小鼠皮下結締組織細胞

ATCC® Number:  CCL-1.4?      
Designations:  A9 (APRT and HPRT negative derivative of Strain L) 
Depositors:   TR Chen 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Mus musculus (mouse) 
Morphology: fibroblast

 A9 小鼠皮下結締組織細胞
Source: Strain: C3H/An
Tissue: subcutaneous connective tissue; areolar and adipose
Disease: normal
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Tumorigenic: Yes 
Antigen Expression: H-2k
Cytogenetic Analysis: modal number = 52; range = 46 to 54; This is a hyperdiploid cell line with 24 to 26 marker chromosomes common to all cells., Nine markers (M1 - M9) are common to L-M(TK-) (see ATCC CCL-1.3) and 16 to 17 are found only in A9. The latter markers include Rb(3.5), Rb(4.15), Rb(5.5), T(8;19), Rb(9.17) and 11 to 12 others., Structurally normal chromosomes 4, 15, 16, 17, 18 and X were absent.
Age:  100 days 
Gender:  male 
Comments: The A9 cell line was isolated by J.W. Littlefield from wild-type Strain L cells.
The cells are sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT).
The cells are resistant to 8-azaguanine (0.003 mg/ml) and 6-thioguanine (0.1 mM).
Propagation:  ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5g/L glucose, 90%; fetal bovine serum, 10%
 A9 小鼠皮下結締組織細胞
Subculturing:  Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove old medium, add fresh 0.25% trypsin, EDTA (0.03%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37C) until cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products: parental cell line:ATCC CCL-1
References: 21409: Littlefield JW. The inosinic acid pyrophosphorylase activity of mouse fibroblasts partially resistant to 8-azaguanine. Proc. Natl. Acad. Sci. USA 50: 568-573, 1963. PubMed: 14067106
21410: Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673
27669: Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365 
 A9 小鼠皮下結締組織細胞